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 หัวเรื่อง:ผลของแสงและความชื้นสัมพัทธ์ในการเพาะเลี้ยงเนื้อเยื่อหน่อไม้ฝรั่งก่อนนำออกปลูก ผู้เขียน: ดร.สุรวิช วรรณไกรโรจน์, รองศาสตราจารย์ ,  Sirilak Kaewsuralikhit สื่อสิ่งพิมพ์:pdf AbstractAsparagus shoots, in experiment 1, were cultured on an MS medium supplemented with 0.04 mg/l IBA and 60 g/l sucrose under white light of 28, 37 and 44 ?mol/m2/s photosynthetic photon flux density (PPFD), blue light of 21,29 and 40 ?mol/m2/s PPFD and red light of 12, 16 and 25 ?mol/m2/s PPFD. The best condition for root induction was under 25 ?mol/m2/s PPFD of red light. After transplanting for 6 weeks, all rooted plants survived. The tallest plantlets and the longest root length were recorded from those previously under white light of 37 and 28 ?mol/m2/sPPFD, respectively. The number of shoot/clump and the whole plant fresh weight were unaffected by light conditions. In another experiment, asparagus shoots were cultured on an MS medium supplemented with 0.04 mg/ l IBA and 60 g/l sucrose in the close chamber inwhich relative humidity (RH) was controlled at 32, 56 and 90% Each culture vessel had a hole either 0, 0.5 or 1.0 cm diameter on its closure. After transplanting for 6 weeks, the tallest plantlets of 22.83 cm were obtained from those previously in the culture vessels with no hole on their closures under 32% RH. The highest fresh weight of 4.81 gm were recorded from those previously in the culture vessel with a 0.5 cm hole on its closure under 32% RH. The highest fresh weight of plantects of 4.81 gm was recorded from those previously in the culture vesses with a 0.5 cm hole on their closures under 32% RH. The root length and the numder of shoot/clump and the whole plant fresh weight of the plantlets were not affected by the RH and the vessel closure conditions. |
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